Endoplasmic Reticulum Enrichment Extraction Kit from Novus Biologicals, a Bio-Techne Brand

Product Specs
ItemEndoplasmic Reticulum Enrichment Extraction Kit
CompanyNovus Biologicals, a Bio-Techne Brand
Price Pricing Info Supplier Page View Company Product Page
Catalog NumberNBP2-29482
Quantity1 Kit
TargetEndoplasmic Reticulum Enrichment
Brand/TrademarkNovus Biologicals, a Bio-Techne Brand
ApplicationsWestern Blot, Immunoprecipitation, Immunohistochemistry-Paraffin
ReactivityHuman, Mouse
StorageStorage of components varies. See protocol for specific instructions

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Novus Biologicals, a Bio-Techne Brand

United States: 10771 E Easter Ave

Centennial, Colorado 80112

United States

Phone:
303-730-1950
888-506-6887

Email: novus@novusbio.com

Company Profile

Website: http://www.novusbio.com

(1) The citrate transporters SLC13A5 and SLC25A1 elicit different metabolic responses and phenotypes in the mouseCommunications biologySeptember 9, 2023Fernandez-Fuente G, Overmyer KA, Lawton AJ, Kasza I, Shapiro SL, Gallego-Muñoz P, Coon JJ, Denu JM, Alexander CM, Puglielli L.and immunoprecipitation Total ER isolation was prepared using a commercially available ER Enrichment kit (Novus Biologicals, #NBP2-29482), according to the manufacturer protocol (see also32). Briefly, 0.5 g of liver tissue was homogenized

(2) Lithium ameliorates Niemann-Pick C1 disease phenotypes by impeding STING/SREBP2 activationiScienceMay 19, 2023Han S, Wang Q, Song Y, Pang M, Ren C, Wang J, Guan D, Xu W, Li F, Wang F, et al.were used for ER membrane isolation using the Endoplasmic Reticulum Enrichment Extraction Kit (Novus, NBP2-29482, Centennial, CO). Cholesterol was then extracted from isolated ER membranes, and the amount of total

(3) A druggable copper-signalling pathway that drives inflammationNatureMay 1, 2023Solier S, Müller S, Cañeque T, Versini A, Mansart A, Sindikubwabo F, Baron L, Emam L, Gestraud P, Pantoș GD, et al.reticula were isolated using the Endoplasmic Reticulum Enrichment Extraction Kit (Novus Biologicals, NBP2-29482) according to the manufacturer’s instructions. In brief, 500 µl of 1× isosmotic homogenization buffer

(4) Assessment of HDAC Inhibitor-Induced Endoplasmic Reticulum (ER) StressMethods in molecular biology (Clifton, N.J.)January 1, 2023Halilovic M, Marx-Blümel L, Marx C, Buder K, Beck JF, Sonnemann J.

(5) Iron Overload via Heme Degradation in the Endoplasmic Reticulum Triggers Ferroptosis in Myocardial Ischemia-Reperfusion InjuryJACC. Basic to translational scienceAugust 1, 2022Miyamoto HD, Ikeda M, Ide T, Tadokoro T, Furusawa S, Abe K, Ishimaru K, Enzan N, Sada M, Yamamoto T, et al.was extracted from the myocardium with the use of an Endoplasmic Reticulum Enrichment Extraction Kit (NBP2-29482, Novus Biologicals). Briefly, frozen myocardium (∼50 mg) was first washed in 1 mL PBS. Subsequently,

(6) ATase inhibition rescues age-associated proteotoxicity of the secretory pathwayCommunications biologyFebruary 25, 2022Murie M, Peng Y, Rigby MJ, Dieterich IA, Farrugia MA, Endresen A, Bhattacharyya A, Puglielli L.

(7) NEK2 inhibition triggers anti-pancreatic cancer immunity by targeting PD-L1Nature communicationsJuly 27, 2021Zhang X, Huang X, Xu J, Li E, Lao M, Tang T, Zhang G, Guo C, Zhang X, Chen W, et al.BD Biosciences), DAB Chromogen kit (BDB2004, Biocare), Duo-link kit (DUO92101, Sigma-Aldrich), ER enrichment kit (NBP2-29482, Novus Biologicals), Pierce GST Protein Interaction Pull-Down Kit (21516, Thermo Scientific), LIVE/DEAD™

(8) Secretion of signal peptides via extracellular vesiclesBiochemical and biophysical research communicationsJune 30, 2021Ono K, Niwa M, Suzuki H, Kobayashi NB, Yoshida T, Sawada M.

(9) Deubiquitinating enzyme OTUB1 promotes cancer cell immunosuppression via preventing ER-associated degradation of immune checkpoint protein PD-L1Cell death and differentiationJune 1, 2021Zhu D, Xu R, Huang X, Tang Z, Tian Y, Zhang J, Zheng X.

(10) Translocation of HSP47 and generation of mitochondrial reactive oxygen species in human neuroblastoma SK-N-SH cells following electron and X-ray irradiationArchives of biochemistry and biophysicsMay 30, 2021Indo HP, Ito H, Nakagawa K, Chaiswing L, Majima HJ.

(11) Regulation of Wnt/PCP signaling through p97/VCP-KBTBD7-mediated Vangl ubiquitination and endoplasmic reticulum-associated degradationScience advancesMay 1, 2021Feng D, Wang J, Yang W, Li J, Lin X, Zha F, Wang X, Ma L, Choi NT, Mii Y, et al.enrichment ER fractions were isolated using the Endoplasmic Reticulum Enrichment Kit (Novus Biologicals, NBP2-29482) according to the manufacturer’s instructions. The CHO cells in a 10-cm dish were transfected with the

(12) Endoplasmic reticulum acetyltransferases Atase1 and Atase2 differentially regulate reticulophagy, macroautophagy and cellular acetyl-CoA metabolismCommunications biologyApril 12, 2021Rigby MJ, Lawton AJ, Kaur G, Banduseela VC, Kamm WE, Lakkaraju A, Denu JM, Puglielli L.prepared as previously described17 using the Endoplasmic Reticulum Enrichment Extraction Kit (Novus; # NBP2-29482). For immunoprecipitation, the total ER pellet was resuspended in kit-provided 1X suspension buffer

(13) ATF4 leads to glaucoma by promoting protein synthesis and ER client protein loadNature communicationsNovember 5, 2020Kasetti RB, Patel PD, Maddineni P, Patil S, Kiehlbauch C, Millar JC, Searby CC, Raghunathan V, Sheffield VC, Zode GS.system. Total ER isolation Total ER fractions were isolated from GTM cells using ER-enrichment kit (cat # NBP2-29482, Novus Biologicals, LLC). GTM3 cells were grown on 10 cm dishes and treated with plasmids expressing

(14) Isolation, Purity Assessment, and Proteomic Analysis of Endoplasmic ReticulumMethods in molecular biology (Clifton, N.J.)January 1, 2020Wang X, Komatsu S.

(15) IL-6/JAK1 pathway drives PD-L1 Y112 phosphorylation to promote cancer immune evasionThe Journal of clinical investigationJuly 15, 2019Chan LC, Li CW, Xia W, Hsu JM, Lee HH, Cha JH, Wang HL, Yang WH, Yen EY, Chang WC, et al.

(16) Insulin prevents and reverts simvastatin-induced toxicity in C2C12 skeletal muscle cellsScientific reportsMay 15, 2019Sanvee GM, Bouitbir J, Krähenbühl S.

(17) Metformin Promotes Antitumor Immunity via Endoplasmic-Reticulum-Associated Degradation of PD-L1Molecular cellAugust 16, 2018Cha JH, Yang WH, Xia W, Wei Y, Chan LC, Lim SO, Li CW, Kim T, Chang SS, Lee HH, et al.nuclear protein. Endoplasmic reticulum (ER) microsomal fractions were prepared by ER enrichment kit (NBP2–29482, Novus Biologicals, Littleton, CO). All experiments were performed according to per manufacturer’s instruction.

(18) Subcellular Proteomics: Application to Elucidation of Flooding-Response Mechanisms in SoybeanProteomesFebruary 27, 2018Komatsu S, Hashiguchi A.

(19) Vitamin C alters the amount of specific endoplasmic reticulum associated proteins involved in lipid metabolism in the liver of mice synthesizing a nonfunctional Werner syndrome (Wrn) mutant proteinPloS oneJanuary 1, 2018Aumailley L, Roux-Dalvai F, Kelly I, Droit A, Lebel M.

(20) O-GlcNAc on NOTCH1 EGF repeats regulates ligand-induced Notch signaling and vascular development in mammalseLifeApril 11, 2017Sawaguchi S, Varshney S, Ogawa M, Sakaidani Y, Yagi H, Takeshita K, Murohara T, Kato K, Sundaram S, Stanley P, et al.ER fraction was obtained using Endoplasmic Reticulum Enrichment Extraction Kit (Novus Biologicals [NBP2-29482]) from parental HEK293T cells or EOGT-null cells, and subjected to immunoblotting with EOGT (1:2000

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